Which of the following is NOT a method used in sterilization?

In the context of sterilization methods, the correct choice indicates that UV light is typically not classified as a sterilization technique. While UV light is often used for disinfection, which reduces the number of viable infectious agents, it does not guarantee that all microorganisms, including spores or resistant forms, are completely eradicated.

Sterilization methods, on the other hand, aim to completely eliminate all forms of microbial life, including fungi, bacteria, viruses, and their spores. Methods such as gases (e.g., ethylene oxide), incineration (which uses high temperatures to destroy all microorganisms), and chemicals (such as formaldehyde or certain high-level disinfectants) are recognized techniques for achieving sterilization.

In summary, the correct choice stems from the distinction between disinfection and sterilization, with UV light leading to a reduction in microbial populations rather than total elimination, making it unsuitable for strict sterilization protocols.