What is the limit of resolution in microscopy?

The limit of resolution in microscopy refers specifically to the minimum distance at which two distinct points can be distinguished as separate entities. This concept is crucial in microscopy because it determines the clarity and detail of the images produced. The smaller the limit of resolution, the more detail can be observed, allowing for better visualization of microscopic structures.

In imaging techniques such as light microscopy, resolution is influenced by factors like the wavelength of light used and the numerical aperture of the lens system. Limits of resolution help define the capability of a microscope to separate close-lying features in a specimen; this is essential for accurate observation and analysis of microorganisms and cellular structures.

The other options describe different aspects of microscopy but do not define the limit of resolution accurately. For instance, the distance at which specimens can be viewed may refer to overall viewing capabilities rather than resolution. The distance between the lens and the specimen or between the objective and eyepiece relates more to the physical setup of the microscope rather than the resolving power. Understanding the limit of resolution is vital for properly utilizing microscopy in scientific research and diagnostics.